Serological markers for Leishmania donovani infection in Nepal: agreement between direct agglutination test and rK39 ELISA
نویسندگان
چکیده
منابع مشابه
Qualitative and Semi-Quantitative Comparison of an Rk39 Strip Test and Direct Agglutination Test for Detection of Anti-Leishmania Donovani Antibodies in the Sudan
Background: Until now, the comparison of the rK39 strip test (RKT) and direct ag-glutination test (DAT) for detection of visceral leishmaniasis (VL) is exclusively based on either positive or negative qualification of the reaction outcome. Objective: In this study, we compared the diagnostic performance of RKT and DAT for VL both qualitatively and semi-quantitatively. Methods: For comparison ba...
متن کاملqualitative and semi-quantitative comparison of an rk39 strip test and direct agglutination test for detection of anti-leishmania donovani antibodies in the sudan
background: until now, the comparison of the rk39 strip test (rkt) and direct ag-glutination test (dat) for detection of visceral leishmaniasis (vl) is exclusively based on either positive or negative qualification of the reaction outcome. objective: in this study, we compared the diagnostic performance of rkt and dat for vl both qualitatively and semi-quantitatively. methods: for comparison ba...
متن کاملComparative evaluation of direct agglutination test, rK39 and soluble antigen ELISA and IFAT for the diagnosis of visceral leishmaniasis.
Five serological tests for the diagnosis of visceral leishmaniasis (VL) were compared: a direct agglutination test (DAT) based on freeze-dried antigen (DAT-fd); a locally produced DAT (DAT-LPC); an IgG ELISA against rK39 (ELISA-rK39); an IgG ELISA for Leishmania chagasi (ELISA-L. chagasi); and an IgG IFAT against L. chagasi. Serum samples from 88 patients with VL, 20 non-infected individuals an...
متن کاملLymphatic leishmaniasis--first case report from Nepal.
We report a case of exclusive involvement of lymph node in leishmaniasis presenting as generalized lymphadenopathy. The diagnosis of lymphatic leishmaniasis was confirmed by the presence of Leishmania donovani body in fine needle aspiration cytology, positive direct agglutination test and anti-rK39 antibodies. The bone marrow aspiration was negative for Leishmania donovani body. This is the fir...
متن کاملrK39 enzyme-linked immunosorbent assay for diagnosis of Leishmania donovani infection.
The rK39 enzyme-linked immunosorbent assay (ELISA) was compared with the direct agglutination test (DAT) for Leishmania donovani infection in the Sudan. rK39 ELISA proved more sensitive than DAT in diagnosis of kala-azar (93 and 80%, respectively); both tests may remain positive up to 24 months after treatment. For patients with post-kala-azar dermal leishmaniasis and individuals with subclinic...
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ژورنال
عنوان ژورنال: Tropical Medicine & International Health
سال: 2010
ISSN: 1360-2276
DOI: 10.1111/j.1365-3156.2010.02631.x